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primary antibodies against c met  (Proteintech)


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    Proteintech primary antibodies against c met
    Primary Antibodies Against C Met, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+c+met/c-Met+(Cytoplasmic)+Antibody/pmc12540219-84-10-14
    Average 94 stars, based on 66 article reviews
    primary antibodies against c met - by Bioz Stars, 2026-09
    94/100 stars

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    Blocking Assay:

    Article Title: Study on the in vitro and in vivo killing effects of PD-1 antibody-secreting c-Met-targeted CAR-T cells on esophageal cancer cell line ECA109
    Article Snippet: .. Following deparaffinization, rehydration, cell permeabilization, antigen retrieval, and serum blocking, primary antibodies against c-Met (proteintech# 25869-1-AP) and PD-L1 (proteintech# 17952-1-AP) were diluted 1:2000 and applied to the slides. ..



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    Expression of key proteins in Wnt signalling pathway in ovarian cancer cells. A, The expression levels <t>of</t> <t>Wnt5a,</t> p‐β‐catenin, β‐catenin, Met and C‐Jun in SKOV3 cells were quantified by image analysis of the Western blot bands. The expression of <t>GAPDH</t> in each group was taken as intrinsic controls, and relative expressions of each protein were calculated. B, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in A2780 cells were quantified by image analysis of the Western blot bands. Values represented the Mean ± SD from three independent experiments. * P < .05, ** P < .01 vs Control. C, Diagram showed the effect of IL‐8 on the migration ability of ovarian cancer cells. IL‐8 up‐regulated the Wnt signalling ligand Wnt5a expression and subsequently activated a β‐catenin–dependent activation of Wnt pathway, which increased the expression of Wnt pathway cascade protein Met and c‐Jun, to finally induce a EMT process to promote the migration of ovarian cancer cells
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    Expression of key proteins in Wnt signalling pathway in ovarian cancer cells. A, The expression levels <t>of</t> <t>Wnt5a,</t> p‐β‐catenin, β‐catenin, Met and C‐Jun in SKOV3 cells were quantified by image analysis of the Western blot bands. The expression of <t>GAPDH</t> in each group was taken as intrinsic controls, and relative expressions of each protein were calculated. B, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in A2780 cells were quantified by image analysis of the Western blot bands. Values represented the Mean ± SD from three independent experiments. * P < .05, ** P < .01 vs Control. C, Diagram showed the effect of IL‐8 on the migration ability of ovarian cancer cells. IL‐8 up‐regulated the Wnt signalling ligand Wnt5a expression and subsequently activated a β‐catenin–dependent activation of Wnt pathway, which increased the expression of Wnt pathway cascade protein Met and c‐Jun, to finally induce a EMT process to promote the migration of ovarian cancer cells
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    Image Search Results


    Inhibition of FGFR1 activation and downstream signaling by AZD4547 were dependent on the EOC cell lines and chemo-sensitive PDX. AZD4547 was applied for different durations in EOC cells. Phospho-FGFR1 and phosphorylation of downstream signaling proteins including p-AKT and p-ERK were investigated in cell lysates and tumor lysates obtained from AZD4547-treated PDX mice compared with control-treated mice by western blot analysis. β-actin was used as a loading control

    Journal: Cancer Cell International

    Article Title: The anti-tumor effects of AZD4547 on ovarian cancer cells: differential responses based on c-Met and FGF19/FGFR4 expression

    doi: 10.1186/s12935-024-03235-2

    Figure Lengend Snippet: Inhibition of FGFR1 activation and downstream signaling by AZD4547 were dependent on the EOC cell lines and chemo-sensitive PDX. AZD4547 was applied for different durations in EOC cells. Phospho-FGFR1 and phosphorylation of downstream signaling proteins including p-AKT and p-ERK were investigated in cell lysates and tumor lysates obtained from AZD4547-treated PDX mice compared with control-treated mice by western blot analysis. β-actin was used as a loading control

    Article Snippet: The blots were probed using primary antibodies against FGFR1, FGFR2 (Santa Cruz Biotechnology, USA), FGFR3, FGFR4 (Abcam), phospho-FGFR1 (p-FGFR1), total-c-Met, phospho-c-Met (p-c-Met), total-AKT, phospho-AKT (p-AKT), total-ERK, phospho-ERK (p-ERK) (Cell Signaling Technology), or β-actin (Santa Cruz Biotechnology).

    Techniques: Inhibition, Activation Assay, Phospho-proteomics, Control, Western Blot

    Expression of key proteins in Wnt signalling pathway in ovarian cancer cells. A, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in SKOV3 cells were quantified by image analysis of the Western blot bands. The expression of GAPDH in each group was taken as intrinsic controls, and relative expressions of each protein were calculated. B, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in A2780 cells were quantified by image analysis of the Western blot bands. Values represented the Mean ± SD from three independent experiments. * P < .05, ** P < .01 vs Control. C, Diagram showed the effect of IL‐8 on the migration ability of ovarian cancer cells. IL‐8 up‐regulated the Wnt signalling ligand Wnt5a expression and subsequently activated a β‐catenin–dependent activation of Wnt pathway, which increased the expression of Wnt pathway cascade protein Met and c‐Jun, to finally induce a EMT process to promote the migration of ovarian cancer cells

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: IL‐8 promotes cell migration through regulating EMT by activating the Wnt/β‐catenin pathway in ovarian cancer

    doi: 10.1111/jcmm.14848

    Figure Lengend Snippet: Expression of key proteins in Wnt signalling pathway in ovarian cancer cells. A, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in SKOV3 cells were quantified by image analysis of the Western blot bands. The expression of GAPDH in each group was taken as intrinsic controls, and relative expressions of each protein were calculated. B, The expression levels of Wnt5a, p‐β‐catenin, β‐catenin, Met and C‐Jun in A2780 cells were quantified by image analysis of the Western blot bands. Values represented the Mean ± SD from three independent experiments. * P < .05, ** P < .01 vs Control. C, Diagram showed the effect of IL‐8 on the migration ability of ovarian cancer cells. IL‐8 up‐regulated the Wnt signalling ligand Wnt5a expression and subsequently activated a β‐catenin–dependent activation of Wnt pathway, which increased the expression of Wnt pathway cascade protein Met and c‐Jun, to finally induce a EMT process to promote the migration of ovarian cancer cells

    Article Snippet: Followed the blocking with 5% fat‐free milk, the membrane was incubated with primary antibodies against E‐cadherin, vimentin, Wnt5a, p‐β‐catenin, β‐catenin, Met, C‐Jun and GAPDH (all from Cell Signaling Technology, Inc) overnight at 4°C and then incubated with a secondary antibody for 1 hours.

    Techniques: Expressing, Western Blot, Control, Migration, Activation Assay